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. 2011 Jun 16;6(6):e20859. doi: 10.1371/journal.pone.0020859

Figure 2. Silencing and overexpression of Gal-3 in pancreatic cancer cell lines.

Figure 2

(A) Transient knockdown of Gal-3 was performed by transient transfection of multiple cell lines using two different Gal-3 specific siRNA sequences (siGal-3 1 and 2) or a non-silencing control siRNA (NC). (B) Stable knockdown in S2-007 was performed using shRNA expression constructs. Three Gal-3 specific shRNA transfected clones (shGal-3 1, 2 and 3) and three nonsilencing control shRNA transfected clones (shC 1, 2 and 3) were chosen for further analysis. (C) Stable overexpression of Gal-3 was achieved by transfection of PaTu 8988t cells with a Gal-3 expression vector (pGal-3 1, 2 and 3) or a control vector (pC 1 and 2). U denotes untransfected cells. Total cell lysates were analyzed by qRT-PCR (upper panels) and Western blot (lower panels) for Gal-3 expression levels. Gal-3 mRNA levels were determined relative to RPLP0. siGal-3 1 and 2 were normalized to NC. β-actin was used as loading control for the Western blots.