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. 2011 Jun 16;6(6):e20859. doi: 10.1371/journal.pone.0020859

Figure 6. Inconsistent effect of Gal-3 knockdown and overexpression on cell migration as measured by Boyden-chamber assay.

Figure 6

Transiently Gal-3 silenced cell lines (A), stably Gal-3 silenced S2-007 cell clones (B) or stably Gal-3 overexpressing PaTu 8988t cell clones (C) were cultured in Boyden-chamber inserts for 2 or 4 h. Cells migrating through the pores of the inserts along a fetal calf serum gradient were fixed, stained methylene blue and counted using light microscopy. Numbers of migrated cells transiently transfected with different Gal-3 specific siRNA (siGal-3 1 and 2) were normalized to control siRNA transfected cells (NC). Migrated stable Gal-3 knockdown cells (shGal-3 1, 2 and 3) and stable Gal-3 overexpressing cells (pGal-3 1, 2 and 3) were normalized to the mean of control vector transfected stable cell clones (shC 1, 2, 3 and pC 1, 2) respectively. Data includes a minimum of three independent experiments. * Indicates p<0.05 as compared with control siRNA transfected cells (double-sided unpaired t-test).