Figure 6. Antibody responses in mice induced by the mono-allelic and bi-allelic PfAMA1 vaccine constructs.
6 week old female BALB/c mice (n = 6) were primed with 1×109 vp of each of the adenovirus constructs i) ChAd63-biallelic, ii) AdHu5-biallelic and iii) AdHu5-PfAMA1 (3D7). Groups i) and ii) were boosted at day 56 with 1×107 pfu of MVA-biallelic and group iii) was boosted with the mono-allelic MVA-PfAMA1 (3D7). The total IgG response was measured by ELISA in the serum at day 14, 55 and 70 against both the 3D7 (A) and FVO (B) alleles. The median and the range of the endpoint titer (log 10) are shown in the figure. There were significant differences between the ELISA titres at day 14 by one-way ANOVA: 3D7 PfAMA1 ChAd63-biallelic versus AdHu5-monoallelic P <0.001; FVO PfAMA1 ChAd63-biallelic versus AdHu5-biallelic P <0.05. The MVA boost significantly increased IgG titres against both alleles in all groups (* P <0.05 by Wilcoxon signed rank test).