Table 3. Cytotoxic effect [IC50 (µg/ml)] of Phyllanthus extracts and standard drugs, against two cancer (A549, MCF-7) and two normal cell lines (NL20, 184B5) after 72 hours incubation.
| IC50 (µg/ml) ± SEM | ||||||
| Cancer Cell Lines | Normal Cell Lines | |||||
| Solvents | A549 | MCF-7 | NL20 | 184B5 | ||
| Plant Extracts | P. niruri (P.n) | Aqueous | 466.7±41.63 | 179.7±0.58 | >500 | >500 |
| Methanolic | 128.3±17.56 | 62.3±9.07 | >500 | >500 | ||
| P. urinaria (P.u) | Aqueous | 215.0±21.79 | 139.3±1.16 | >500 | >500 | |
| Methanolic | 69.0±11.53 | 48.7±10.02 | >500 | >500 | ||
| P. watsonii (P.w) | Aqueous | 198.3±10.41 | 104.0±10.39 | >500 | >500 | |
| Methanolic | 61.3±16.17 | 49.0±8.19 | >500 | >500 | ||
| P. amarus (P.a) | Aqueous | 240.0±26.46 | 156.7±5.77 | >500 | >500 | |
| Methanolic | 126.7±7.64 | 56.3±6.66 | >500 | >500 | ||
| Standard Drugs | Cisplatin | 7.6±1.10 | 1.4±0.54 | 0.9±0.05 | 3.0±0.03 | |
| Doxorubicin | 0.6±0.08 | 0.4±0.05 | 0.3±0.02 | 0.6±0.03 | ||
| Fraction 1 | P. niruri (P.n) | Aqueous | 380.0±18.03 | 438.3±11.55 | 266.7±41.93 | 283.3±25.17 |
| P. urinaria (P.u) | Aqueous | >500 | >500 | >500 | 231.7±18.93 | |
| P. watsonii (P.w) | Aqueous | 395.0±8.66 | 376.7±2.89 | 241.7±20.21 | 230.0±26.46 | |
| P. amarus (P.a) | Aqueous | >500 | >500 | >500 | >500 | |
| Fraction 2 | P. niruri (P.n) | Aqueous | 228.3±5.77 | 81.7±16.07 | 108.3±5.77 | 230.0±50.74 |
| P. urinaria (P.u) | Aqueous | 225.0±13.23 | 61.7±12.58 | 95.0±5.00 | 230.0±13.23 | |
| P. watsonii (P.w) | Aqueous | 225.0±43.30 | 46.7±10.41 | 105.0±5.00 | 201.7±20.21 | |
| P. amarus (P.a) | Aqueous | 264.3±45.24 | 70.0±17.32 | 106.7±7.64 | 213.3±54.85 | |
Data is expressed as a mean of three independent experiments ± Standard Error Mean (SEM).