Table 1. Clustering analysis of GO terms for genes upregulated in fkh mutants.
| Annotation Clustera | Gene Onotology (GO) term | Fold enrichment | P-value |
| 1 (6.74) | Chitin metabolic process | 3.6 | 9.0×10−9 |
| Polysaccharide metabolic process | 3.4 | 1.5×10−10 | |
| Aminoglycan metabolic process | 3.4 | 1.4×10−9 | |
| Polysaccharide binding | 3.0 | 9.2×10−8 | |
| Pattern binding | 3.0 | 9.2×10−8 | |
| Chitin binding | 3.0 | 2.7×10−6 | |
| Chitin binding protein, peritrophin-A | 2.9 | 1.5×10−5 | |
| ChtBD2 | 2.6 | 6.8×10−5 | |
| Carbohydrate binding | 2.4 | 2.4×10−6 | |
| Extracellular region | 1.8 | 3.2×10−7 | |
| 2 (5.52) | Synapse | 3.8 | 8.3×10−6 |
| Cell junction | 3.2 | 1.5×10−5 | |
| Cell junction | 3.0 | 9.8×10−8 | |
| Synapse | 2.9 | 1.0×10−6 | |
| Synapse part | 2.8 | 1.9×10−5 | |
| 3 (4.90) | Structural constituent of cuticle | 3.0 | 1.6×10−7 |
| Structural constituent of chitin-based larval cuticle | 2.9 | 1.1×10−2 | |
| Insect cuticle protein | 2.8 | 4.4×10−6 | |
| Structural constituent of chitin-based cuticle | 2.8 | 4.4×10−6 | |
| Structural molecule activity | 1.8 | 9.1×10−6 | |
| 4 (4.04) | Basement membrane | 7.8 | 1.0×10−4 |
| Extracellular matrix part | 7.2 | 1.8×10−4 | |
| Extracellular matrix | 4.0 | 4.2×10−6 | |
| Proteinaceous extracellular matrix | 4.0 | 1.0×10−5 | |
| Extracellular region part | 1.9 | 7.9×10−3 | |
| 5 (3.98) | Fatty acid biosynthetic process | 5.1 | 2.7×10−5 |
| Lipid synthesis | 4.4 | 8.9×10−3 | |
| Fatty acid metabolic process | 4.1 | 9.2×10−6 | |
| Organic acid biosynthetic process | 3.6 | 2.1×10−5 | |
| Carboxylic acid biosynthetic process | 3.6 | 2.1×10−5 | |
| Lipid biosynthetic process | 2.3 | 1.3×10−3 | |
| 6 (3.60) | Plasma membrane part | 2.0 | 3.1×10−8 |
| Integral to plasma membrane | 1.6 | 2.1×10−2 | |
| Intrinsic to plasma membrane | 1.6 | 2.5×10−2 | |
| 7 (3.48) | Muscle | 13 | 1.8×10−5 |
| Striated muscle thin filament | 13 | 1.4×10−4 | |
| Contractile fiber | 10 | 3.3×10−12 | |
| Sarcomere | 9.8 | 1.0×10−8 | |
| Contractile fiber part | 9.7 | 3.1×10−10 | |
| Muscle protein | 9.4 | 2.4×10−9 | |
| Myofibril | 9.2 | 2.5×10−8 | |
| Myofibril assembly | 7.2 | 2.0×10−4 | |
| Skeletal muscle tissue development | 3.7 | 2.2×10−3 | |
| Skeletal muscle organ development | 3.3 | 4.2×10−4 | |
| Striated muscle tissue development | 3.3 | 5.3×10−3 | |
| Striated muscle cell development | 3.2 | 3.1×10−3 | |
| Muscle cell development | 3.2 | 3.1×10−3 | |
| Muscle tissue development | 3.2 | 6.2×10−3 | |
| Striated muscle cell differentiation | 3.1 | 4.1×10−4 | |
| Muscle cell differentiation | 2.9 | 4.6×10−4 | |
| Actomyosin structure organization | 2.7 | 4.4×10−2 | |
| Actin cytoskeleton | 2.3 | 4.6×10−3 | |
| Muscle organ development | 2.1 | 3.4×10−3 | |
| 8 (3.00) | Domain: EF-hand 3 | 6.6 | 2.0×10−5 |
| Domain: EF-hand 4 | 6.1 | 1.8×10−3 | |
| Domain: EF-hand 1 | 4.9 | 9.0×10−5 | |
| Domain: EF-hand 2 | 4.9 | 9.0×10−5 | |
| Calcium-binding region 1 | 4.8 | 7.8×10−4 | |
| Calcium-binding region 2 | 4.8 | 7.8×10−4 | |
| EF hand | 4.8 | 1.6×10−2 | |
| Signal transduction mechanisms / Cytoskeleton / Cell division and chromosome partitioning / General function prediction only | 3.3 | 1.5×10−2 | |
| Calcium | 2.4 | 1.9×10−5 | |
| Calcium ion binding | 2.3 | 4.1×10−7 | |
| EF-Hand 1 | 2.1 | 3.2×10−3 | |
| EF-Hand type | 2.1 | 3.7×10−3 | |
| Calcium-binding EF-hand | 2.1 | 2.2×10−2 | |
| EF hand | 2.1 | 2.2×10−2 | |
| EF-Hand 2 | 2.0 | 1.1×10−2 | |
| EFh | 1.8 | 4.5×10−2 |
David analysis reveals a significant enrichment of eight annotation clusters (based on Gene Ontology terms) in the set of genes upregulated in stage 11 fkh mutant embryos. Shown are all clusters with enrichment scores ≥3.00
a
The enrichment score is shown in parentheses. P<0.05.