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. 2011 Jun 16;6(6):e21059. doi: 10.1371/journal.pone.0021059

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Male zebrafish (N = 35) were squeezed and their body condition (K⁼100,000 x weight/(standard length)³ ) determined and correlated with characteristics of the undiluted ejaculated. A) Body condition (K) did not correlate with sperm volume or B) motility, but C) increasing body condition (K) correlated with a decreasing sperm concentration. Analysis suggested that there was no correlation between body condition and sperm motility, nor body condition and ejaculate volume (Fig. 1 A and B; P>0.05, Linear regression). However, paradoxically, fish with increasing body condition, produced less concentrated sperm (Fig. 1C; P<0.05). In fact, the most extreme body condition values (1.73 and 2.47) demonstrated a 75% decrease in sperm concentration.