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The p7 and p61 viral promoters drive the expression of the NS and VP genes, respectively. In p7NS1-GFP, GFP is fused to the NS1 gene. In p7NS1-Intron-GFP, the chemical intron sequence is inserted in the AgeI site of the NS1-GFP fusion. RNAi plasmids pAnSI-1, pAnSI-2, pAeSI-1 and pAeSI-2, contain the artificial shRNA expression cassette, which was cloned into the MluI and NheI sites of the intron.
