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. 2011 Jun 16;7(6):e1002136. doi: 10.1371/journal.pgen.1002136

Figure 1. Survivors of the untimely initiation of DNA replication experiments have aberrant chromosomes.

Figure 1

A, Chromosomal DNA from YST563 (JET1-1 GALp-sld2-11D) survivors from Figure S1 was analyzed using pulsed-field gel electrophoresis. Surviving colonies (survivors 1–17) were recovered on YPAD plates after galactose incubation. Abnormal chromosome bands are indicated with arrowheads, chromosomes with increased band intensity are indicated with filled arrowheads, and chromosomes with different lengths are indicated with open arrowheads. Chr XII bands are not marked because they are unstable even in wild-type cells. B, Quantified profiles of band intensities of A are shown. Abnormal chromosome bands are indicated with arrowheads. *: non-chromosomal signal originated from contaminated dusts.