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. 2011 Jun 16;7(6):e1002136. doi: 10.1371/journal.pgen.1002136

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Tanaka, Araki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Relative GCR values in Table 2 are graphically shown. The GCR rate of RDKY3615 (Wt (SLD2)) is set as one. B, RDKY3615 (Wt (SLD2)), YST1336 (sld2-11D), YST1090 (+vector), YST1737 (+SLD2), YST1739 (+sld2-11D), YST1747 (+sic1N-SLD2) and YST1749 (sic1N-sld2-11D) cells were grown (asynchronous), and aliquots of the culture were arrested in G1 or S phase with alpha factor or HU, respectively. Whole cell extracts were prepared and subjected to western blotting. Sld2 proteins were detected with anti-Sld2 antibody. The loading control shows the corresponding region of the Ponceau-S-stained membrane. *: non-specific background band. Lanes 14, 15, 21 and 22 are enlarged to show the Sic1N-Sld2 bands (bottom).