Table 1. The GCR rate for GALp-SLD2 cells.
Strain | Genotype | GCR rate (×10−10/cell div.) | Relative value*1 | |
Glc (OFF) | Gal (ON) | |||
YST1007 | GALp vector | 1.5 | 0.79 | 1 |
YST1008 | GALp-SLD2 | 0.70 | 11 | 13 |
YST1024 | GALp-sld2-11D | 1.7 | 580 | 730 |
YST1338 | GALp-Db | 0.84 | 0.72 | 0.91 |
YST1062 | GALp-Db-SLD2 | 0.88 | 1.7 | 2.1 |
YST1064 | GALp-Db-sld2-11D | 1.1 | 110 | 140 |
YST1159 | GALp-sic1N | 0.75 | 2.3 | 3.0 |
YST1161 | GALp-sic1N-SLD2 | <1.9*2 | 150 | 190 |
YST1162 | GALp-sic1N-sld2-11D | <1.4*2 | 370 | 460 |
YST1128 | GALp-CDC6 GALp vector | ND*3 | 1.6 | 2.0 |
YST1129 | GALp-CDC6 GALp-SLD2 | <1.2*2 | 340 | 430 |
YST1130 | GALp-CDC6 GALp-sld2-11D | 2.3 | 870 | 1100 |
YST1743 | yel062w::ARS306 GALp vector | 1.8 | 0.89 | 1.1 |
YST1745 | yel062w::ARS306 GALp-SLD2 | <1.8*2 | 100 | 130 |
YST1744 | yel062w::ARS306 GALp-sld2-11D | 1.9 | 400 | 500 |
YST1051 | GALp-DPB11 | 0.78 | 48 | 61 |
*1: The relative value of the GCR rate from the +Gal condition. The GCR rate of the control strain (YST1007) is set as 1.
*2: The number of plates on which mutants appeared was less than half (see Materials and Methods for details).
*3: The GCR rate could not be determined because colonies did not appear on all plates.