Table 2. Genes that elicit ASE cell/lineage defects when knocked-down by RNAi.
Gene Name | Functional Category | Domain/Function | ASEL | >2 ASEL | ASER | >2 ASER | ASE | >2 ASE | Viable | ASE lineage phenotype | Mutant phenotype |
lis-1 | cell/organelle | WD40 | - | wt | +/− | Loss ASEL | |||||
lrg-1 | chromosome | let-99 related (pseudogene) | + | +/− | + | + | ectopic ASEL1 | ||||
par-3 | blastomere | PDZ domain | + | + | + | + | + | ectopic ASEL1 | |||
mei-2 | blastomere | Microtubule regulator (similar to katanin) | + | - | +/− | + | ectopic ASEL2 | ||||
par-2 | blastomere | RING finger | + | + | - | ne | ectopic ASEL2 | Yes | |||
lit-1 | blastomere | Serine/threonine kinase (Wnt signalling) | - | + | + | + | + | ectopic ASER | |||
pie-1 | blastomere | Zinc finger, CCCH-type | + | + | - | + | + | ectopic ASEL2 | |||
ced-3 | signalling | Caspase | + | + | + | + | + | Yes | ectopic ASEL1 | Yes | |
oac-35 | signalling | Integral membrane O-acyltransferase | + | + | - | ne | ectopic ASEL2 | ||||
wwp-1 | signalling | E3 ubiquitin ligase | + | + | + | +/− | + | ectopic ASEL1 | |||
ref-1 | transcription | bHLH | + | wt | + | + | Yes | ectopic ASEL | Yes |
See Materials and Methods for a detailed description of scoring criteria. Abbreviations: ne, not embryonic lethal; wt, wildtype.
also ectopic ASER lineage.
also loss of ASER lineage.