AZD8055/αCD40 induced anti-tumor responses were dependent on IFN-γ. A, Real-time quantitative PCR was used to analyze IL-12, IFN-γ, TNFα, CCL5, CXCL10, and CXCL9 expression in the livers of AZD8055/αCD40 treated tumor-bearing mice on day 17. * p<0.05, ** p<0.01, VC control group versus all other groups. B, Anti-tumor responses induced by AZD8055/αCD40 were evaluated in IFN-γ KO (GKO) mice. Liver nodules were counted on day 17. C–D, Hepatic MNCs were isolated from IFN-γ KO mice on day 14 and analyzed by flow cytometry. Absolute numbers of CD8+ T cells and NK cells and percentage of CD69+ cells among them in IFN-γ KO mice are shown in (C). Absolute number of macrophages was shown in (D). * p<0.05, ** p<0.01, *** p<0.001. All data are representative of two or three independent experiments.