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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Cancer Res. 2011 Apr 20;71(12):4314–4324. doi: 10.1158/0008-5472.CAN-11-0051

Figure 4.

Figure 4

HOXC9 represses the expression of cell cycle genes. A, qRT-PCR analysis of mRNA levels of cyclins, CDK1 and HOXC9 in BE(2)-C cells with or without HOXC9 induction for 5 days (error bars, s.d., n=3). B, Time-course immunoblot analysis of cyclins and CDKs in BE(2)-C cells following HOXC9 induction. α-tubulin levels are shown as loading control. C, ChIP-qPCR analysis showing HOXC9 interaction with the CCNB1 promoter region (−430 to −178) following HOXC9 induction (error bars, s.d., n=3). Percentage of input indicates bound/input × 100. D, Top: schematic representation of the CCNB1 promoter luciferase reporter construct and its mutated version. Bottom: luciferase reporter assays in the absence or presence of a HOXC9-expressing plasmid (error bars, s.d., n=3). Data were analyzed using two-tailed Student’s t-test with p values indicated. E, ChIP-qPCR analysis of H3K4me3 and H3K27me3 levels at the CCNB1 promoter (−231 to +6) in BE(2)-C cells following HOXC9 induction (error bars, s.d., n=3). Percentage of input indicates bound/input × 100.