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hScrt and hASH1-E12 bind specifically to a conserved E-box sequence. hScrt and hASH1-E12 protein produced by IVT were used in an EMSA. The wild type probe contains an optimal Snail binding site (ACAGGTG), whereas the mutant probe contains three nucleotide changes (TTAGGTA). Excess of unlabeled wild type probe (W), amounts indicated by the inclining wedge (0-, 10-, 50-, 100-, 500-, 1,000-, or 2,000-fold), or 2,000-fold excess mutant probe (M) was used as a competitor.
