Skip to main content
. 2001 Mar 13;98(7):4010–4015. doi: 10.1073/pnas.051014098

Figure 5.

Figure 5

hScratch is a nuclear protein that can repress bHLH function. In NCI-H1299 cells, cotransfection of hASH1 and E12 expression vectors activated a luciferase reporter downstream of four E-box motifs. Increasing amounts of full-length hScrt inhibited hASH1-E12 transactivation of the reporter to 32% of control levels (P < 0.001). No effect on reporter activity was seen with the mutant construct lacking residues N-terminal to the five zinc fingers (Δ N terminus) or a mutant hScrt lacking the five zinc fingers (Δ Zinc Fingers) (P > 0.05). Luciferase activity of cells cotransfected without hASH1-E12 was set at 1.0. The data represent averages and standard errors of values obtained from two different experiments performed in duplicate or triplicate. Statistical analysis of a representative experiment was performed by one-way ANOVA followed by the Bonferroni test.