Figure 4. Effect of SNP rs7945931genotypes on human total SORL1-mRNA expression levels in human temporal cortex.
Individuals were genotyped for SNP rs7945931 and fold levels of human total SORL1-mRNA were assayed by real-time RT-PCR using TaqMan technology and calculated relative the geometric mean of SYP- and ENO- mRNAs reference control using the 2−ΔΔCt method. Analysis of the temporal cortex showed that the minor allele carriers ‘+A’ genotypes (AA, AG; n=10) correlate with significantly higher total SORL1-mRNA levels then the non carrier, ‘−A’ genotype (GG; n=41) (p=0.008). The same significant trend was observed in the analysis of SNP rs2298525 (p=0.01).