Figure 2.

PP6 is required for repair of CPT-induced DSBs. (A) U2OS cells were transfected with si-CONTROL or siRNA oligos against PP6 subunits. Transfectants were untreated, treated with CPT (10 mM) for 1 h or washed free of drug after 1 h-CPT treatment and then incubated for 8 h. Cells were collected for neutral comet assays. A representative image of cells under each condition is presented. (B) Quantification of the tail lengths from the experiment for which results are shown in (A). The tail length for each condition was calculated from a minimum of 100 cells for each data point.