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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Apr 7;31(7):1565–1572. doi: 10.1161/ATVBAHA.111.227629

Figure 3.

Figure 3

Effect of IL17 on Plaque Composition. ApoE−/− and IL17/ApoE−/− mice were fed 3 months of high fat diet. Panel A shows Russell-Movat pentachrome staining in the aortic root at 4× and 10× magnification. Black=elastic fibers, yellow=collagen, blue/green=mucins, red=muscle, intense red=fibrinoid. Figures are representative of 4 per group. To analyze inflammatory cell content, flow cytometry of single cell suspensions of whole aortas were performed as shown in panel B. Representative scatter plots are shown on the left, and summary data of n=9–12 mice per group are shown on the right. Data were analyzed using Student's t test. CD45+ cells represent total leukocytes, CD3+ cells represent T cells, CD11b-11c+ cells represent dendritic cells, and CD11b+F4/80+ cells represent macrophages. Panel C shows plaque macrophage content in the aortic root as determined by immunostaining with a Mac3 antibody. Alkaline phosphatase (pink) was used to detect the secondary antibody. Slides were counterstained with hematoxylin. A representative of n=3 per group is shown at 4× and 10× magnification.