Differential effect of abscisic acid (ABA) on colon gene expression in PPAR γ-expressing and T cell-specific PPAR γ deficient mice. PPAR γ flfl;CD4 Cre− (wt) and PPAR γ flfl; CD4 Cre+ (CD4cre) mice were fed ABA-supplemented (100 mg/kg) or control diets for 35 days and challenged with 2.5% dextran sodium sulfate (DSS) for 7 days. Expression of (A) peroxisome proliferator-activated receptor γ (PPAR γ), (B) mucosal vascular addressin cell adhesion molecule 1 (MAdCAM-1), (C) vascular adhesion molecule 1 (VCAM-1), (D) E-selectin, (E) interleukin 6 (IL-6), (F) matrix metalloproteinase 9 (MMP-9), and (G) interferon γ (IFN-γ) was quantified by real-time quantitative RT-PCR. Statistically significant differences (P<0.05) between groups are indicated with different letter subscripts (n=8 mice/group) in three independent experiments. (H) The effect of ABA treatment on colonic gene expression for each genotype was calculated as a relative expression value with respect to the control treatment. Data are represented as mean ± standard error. Points with an asterisk are significantly different (P<0.05). Statistically significant differences (P<0.05) between groups are indicated with an asterisk (n=8 mice/group) in three independent experiments.