Table 1.

Oligonucleotide sequences for quantitative real-time PCR.^*, ^†

Primer	Sequence	Length	Accession Number
βactinF	5′CCCAGGCATTGCTGACAGG3′	141	X03672
βactinR	5′TGGAAGGTGGACAGTGAGGC3′
PPAR γF	5′CAGGCTTGCTGAACGTGAAG3′	117	NM_011146
PPAR γR	5′GGAGCACCTTGGCGAACA3′
E-selectinF	5′CAGCTTTGCATGATGGCGTCT3′	83	NM_011345
E-selectinR	5′GAAGGGTACAGGCGAGTTGG3′
VCAM-1F	5′TCTCCCAGGAATACAACGAT3′	75	NM_011693
VCAM-1R	5′ACAGGTCATTGTCACAGCAC3′
MAdCAM-1F	5′CCCATGGCCACAGCTACCTCA3′	85	NM_013591
MAdCAM-1R	5′CCCTGGCCCTAGTACCCTAC3′
MMP-9F	5′GGTGGCAGCGCACGAGTT3′	128	NM_013599
MMP-9R	5′GGATGCCGTCTATGTCGTCTT3′
IL-6F	5′TTTCCTCTGGTCTTCTGGAG3′	92	NM_031168
IL6-R	5′CTGAAGGACTCTGGCTTTGT3′
IFNγ F	5′GGGGGTGGGGGACAGC3′	140	K00083
IFNγ R	TGGCCCGGAGTGTAGACATC3′

^*F, forward; R, reverse. PCR primer pairs were designed for an optimal annealing temperature of 57°C and product lengths between 78 and 157 base pairs.

^†When plotting threshold cycle versus log starting quantity (pg), standard curves had slopes between −3.1 and −3.7; PCR efficiencies between 92 and 105 and R² above 0.98.