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. Author manuscript; available in PMC: 2012 Mar 19.
Published in final edited form as: Arthritis Rheum. 2011 Feb;63(2):556–567. doi: 10.1002/art.30138

Figure 2. DEK auto-antibodies are detected in synovial fluids from patients with JIA.

Figure 2

(A). Proteins from mock-infected insect cell extracts (negative control) or recombinant histidine-tagged DEK expressed and purified from High5 insect cells (1 µg per lane) were resolved by SDS-PAGE and subjected to immunoblotting with a 1:200 dilution of crude synovial fluids. Shown are Western blots using synovial fluids from 3 different JIA patients that tested positive for DEK-specific auto-antibodies and are representative of 10 out of 13 synovial fluids from JIA patients that were screened. Also shown are results from three non-JIA patients’ synovial fluids that had no-to-very low levels of DEK-specific auto-antibodies and are representative of 6 orthopedic surgery (OS), 1 rheumatoid arthritis (RA), and 2 osteoarthritis (OA) patients’ synovial fluids. (B). Samples were also probed with synovial fluid that had been affinity purified using SulfoLink sepharose columns covalently conjugated with recombinant His-DEK. (C). Insect cell extract and recombinant His-DEK were probed with a monoclonal antibody to DEK as a positive control.