Figure 2.

Diffusion law recorded with sv-FCS. (a) By increasing the area of the excitation spot, the transit times in the autocorrelation curves increase accordingly. Fitting each ACF with an effective two-dimensional diffusion yields τ_D(ω²). From the y-intercept, the diffusion law offset, τ₀, is obtained. (b) Full ACF Monte Carlo simulations were performed for tracers that were stochastically immobilized with exponentially distributed transition kinetics. Simulation data (symbols) follow the theoretical curves (lines). A trapping time of τ_trap = 500 ms was adjusted.