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Standard calibration curves generated from sperm samples between sperm concentration (cells/mL) and absorbance measured at 400 nm by use of a microspectrophotometer. Zebrafish (D. rerio) sperm samples collected by crushing of testis (A) or by stripping of live males (B); Xiphophorus helleri sperm samples collected by crushing of testis (C) or stripping of live males (D). Medaka Oryzias latipes sperm samples collected by crushing of testis (E).
