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Standard curves generated from serial dilutions of three aliquots of pooled zebrafish sperm samples. Identical slope values in the equations (3 × 108) and slight variations in y-intercept value (2 × 107 for replicate 1, and 1 × 107 for replicates 2 and 3) indicated the reproducibility of the method for generating curves from serial dilutions by use of the microspectrophotometer for absorbance measurement and hemocytometer for cell counting.
