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. 2010 Jun;7(2):149–154. doi: 10.1089/zeb.2010.0660

FIG. 2.

FIG. 2.

Specimen in a Corrected Optical Rotational Enclosure (SCORE) imaging of living animals. Zebrafish imaging is facilitated by use of a fluoro-carbon tube for housing. (A) Zebrafish images taken in polymer tubing produce a distorted image (arrows) due to the change in refractive index between the tubing and the air. Cartoon images representing a fish immobilized using traditional techniques on a skewed plane (B), a larval fish within a polymer tube without a corrective solution producing distortion (C), and within a corrective solution to produce a clear image (D). Fluorinated ethylene propylene (FEP) tubing produces a clear image at 50 × (E ) (also see Supplemental Movie S1, available online at www.liebertonline.com), 100 × (F), and 200 × (G) of a 5 day postfertilization (dpf ) larvae. A 5 dpf Tg(gata-1:DsRed)sd2 larvae in FEP tubing produces a clear background-free image at 50 × (H) (also see Supplemental Movie S2, available online at www.liebertonline.com) and 100 × (I). A 5 dpf Tg( fli:eGFP)y1 in FEP tubing produces a clear background-free image at 50 × (J) (also see Supplemental Movie S3, available online at www.liebertonline.com) and 100 × (K).