FIG. 4.

SCORE imaging of fluorescent, living zebrafish. Imaging SEC0124 of a 5 dpf mRFP-labeled protein trap zebrafish housed in FEP tubing. (A) Sagittal image of an mRFP-labeled protein trap zebrafish shows enhancement in specific locations in the neural region as well as a bright kidney (Arrow). (B) Coronal image of an mRFP-labeled protein trap zebrafish includes ubiquitous neural expression with enhancement in specific locations. (C) Sagittal image of an mRFP-labeled protein trap zebrafish shows a general neural haze with enhancement in specific locations at 100 × magnification (also see Supplemental Movie S5 [showing the spatial location of RFP patterning], available online at www.liebertonline.com).