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Refractive Indices of Polymer Tubing Used
| Solution | Refractive index | Comments about use |
|---|---|---|
| Water (surrounding fluid) | 1.3415 | Used to cover tubing; matched for tubing |
| FEP | 1.33816 | Recommended for live embryos |
| PFA | 1.3517 | Works well for live embryos |
| PTFE | 1.35–1.3816 | Not recommended |
FEP, fluorinated ethylene propylene; PFA, perfluoroalkoxy; PTFE, polytetrafluoroethylene.
