Figure 6.

Significant loss of RAF1 binding affinity for RAS mutants. Binding of RAF1-RBD (increasing concentrations as indicated) to mantGppNHp-bound RAS (0.2 µM) was measured using fluorescence polarization. On the panel A, the respective concentration-dependent measurements of RAS^wt and a representative RAS mutant (p.G60R) are shown. On panel B, the dissociation constants (K_d) of all RAS proteins are illustrated. RAS^wt, RAS^G12V, and RAS^F28L were included as controls. Data obtained with another RAS effector, RALGDS are shown in Supp. Fig. S5.