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. Author manuscript; available in PMC: 2011 Jun 17.
Published in final edited form as: Biomaterials. 2009 Dec 4;31(8):2153–2162. doi: 10.1016/j.biomaterials.2009.11.074

Fig. 2.

Fig. 2

Neurofilament staining of fetal porcine retina. A–D: E50 retinas co-cultured for 9 (A, C) and 16 (B, D) days in vitro with PGS membranes coated with PCL (A, B) or laminin-PCL blend (C, D) nanofibers. Neurofilament staining is absent in the retinas co-cultured with nanofiber coated PGS membranes. E–F: E49 retinas cultured for 7 (E) and 14 (F) days in vitro. Neurofilament positive ganglion cells are visible in the ganglion cell layer (E). However, neurofilament staining is less evident following longer culture in vitro (F). G–H: E60 (G) and E70 (H) in vivo retina specimens. Neurofilament positive axons are visible in the nerve fiber layer (G). Intensely labeled neurofilament positive axons in the nerve fiber layer are visible, and horizontal cells in the outer part of the INL are also labeled (H). Neurofilament staining is less pronounced in in vitro specimens (E–F) compared to in vivo specimens (G– H). VITR, vitreal (inner) aspect, SCL, sclera (outer) aspect. Scale bar = 50 microns.