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. Author manuscript; available in PMC: 2011 Jun 17.
Published in final edited form as: AJR Am J Roentgenol. 2010 Aug;195(2):341–349. doi: 10.2214/AJR.09.3672

Fig. 5. Construction and in vitro characterization of BxPC-3 hNIS-IRES-Em cells.

Fig. 5

A,. Schematic representation of pHR-SIN-hNIS-IRES-Em, a self-inactivating, bi-cistronic lentiviral transfer vector used to create BxPC-3 cells which stably express the human sodium iodide symporter and a green fluorescent protein under control of spleen focus forming virus (SFFV) promoter. LTR = long terminal repeat, RRE = HIV rev response element, cPPT = central polypurine tract, hNIS, human sodium iodide symporter (SLC5A5), IRES = internal ribosome entry site, EmGFP = emerald enhanced green fluorescent protein, WPRE = woodchuck hepatitis virus posttranscriptional regulatory element, ΔU3 = deletion of U3 region. B, Graph shows Western blot of BxPC-3-hNIS-IRES-Em cells (26 µg total protein; lane 1) and control, nontransduced BxPC-3 cells (45 µg total protein; lane 2). C, Graph shows iodide uptake of BxPC-3 control cells and BxPC-3-hNIS-IRES-Em cells; 150,000 cells were incubated for 45 minutes at 37 °C in the presence of 17.8 kBq 125I-Na. on basis of cell volume of 3.566 pL, the BxPC-3-hNIS-IRES-Em cells accumulated 125I to a level 101 times that of external concentration.