Table 1.
Characteristics of transgenic mouse lines
| Mouse line | Gene copy number* | PrP RNA† (spleen) | PrP‡ (spleen) | PrP§ (leukocytes) |
|---|---|---|---|---|
| Prnp+/+ | 2 | 1 | 1 | 1 |
| Prnp+/0 | 1 | ND | 0.5 | ND |
| TgN306 hemizygous | 5 | 0.55 | ND | 0.4 |
| TgN306 homozygous | 10 | ND | 0.8 | ND |
| TgN431 hemizygous | 15 | ND | 50–100 | 10–20 |
ND, not determined.
*
Relative to wild type; determined by Southern blot analysis.
†
Relative to wild type; determination of PrP mRNA by quantitative Northern blot analysis.
‡
Relative to wild type; determination of PrPC by densitometry of Western blots.
§
Relative to wild type; determined by FACS analysis.