FIGURE 2.

Mapping of GrB cleavage motifs in human TAL. A, Locations of GrB recognition motifs are underlined, and sites of cleavage are indicated by vertical arrows. CTL epitope residues 168–176 are italicized. B, Digestion of TAL-WT and mutated TAL carrying an aspartic-to-alanine substitution at aa position 27 (TAL-D27A). A total of 100 ng of recombinant protein was incubated without (−) or with (+) 150 nM GrB for 1 h at 37°C and detected by anti-TAL Ab 169, using Western blot.