Skip to main content
. Author manuscript; available in PMC: 2011 Nov 13.
Published in final edited form as: Cell. 2011 May 13;145(4):607–621. doi: 10.1016/j.cell.2011.03.043

Figure 1. Class IIa HDACs Are Regulated by LKB1-dependent Kinases and Metformin Treatment in Liver.

Figure 1

(A) Clustal alignment of Class IIa HDACs showing sequence conservation on established phosphorylation sites matching the optimal AMPK motif.

(B) Primary mouse hepatocytes or mouse livers lysates infected with adenoviruses bearing indicated shRNAs and immunoblotted with indicated antibodies (full description in Supplemental Supporting Text).

(C) Lysates of HepG2 or Huh7 cells transfected with indicated siRNA pools and treated with either 2mM Phenformin or vehicle for 1hr and subjected to immunoblotting.

(D) Immunoblot of lysates from murine livers from LKB1+/+ or LKB1lox/lox mice deleted for hepatic LKB1 and treated with either 250mg/kg metformin, or saline alone for 1h.

See also Figure S1.