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. Author manuscript; available in PMC: 2011 Nov 13.
Published in final edited form as: Cell. 2011 May 13;145(4):607–621. doi: 10.1016/j.cell.2011.03.043

Figure 5. Class I HDAC3 is Recruited by Class IIa HDACs to Deacetylate Foxo.

Figure 5

(A) ChIP analysis on primary hepatoyctes transduced with control scramble or HDAC4/5/7 shRNAs and assessed for HDAC3 association on Foxo binding sites within G6Pase or PCK1 or the housekeeping TFIIB promoter following 1h treatment with 100nM Glucagon. (n= 4) *p<0.05

(B) HEK293T cells transfected with a FLAG-HDAC3 and GFP-HDAC5 as indicated and treated with forskolin or vehicle for 1h and then immunoprecipitated with anti-FLAG tag antibodies. Immunoprecipitates and input cell lysates were blotted with indicated antibodies.

(C) In vitro deacetylation assays were performed on recombinant GST-FOXO1, pre-acetylated in vitro with a recombinant fragment of p300. GST-FOXO1 acetylation is detected using the Foxo1 K242/245 acetylation specific antibody. Recombinant HDAC3 or HDAC3 complexed with Ncor was used at varying concentrations. Recombinant SIRT1 used as positive control.

(D) ChIP analysis on primary hepatoyctes transduced with control scramble or HDAC4/5/7 shRNAs and assessed for Foxo1 on G6Pase or PCK1 promoters following 1h treatment with 100nM Glucacon. (n= 4) *p<0.05

Data are shown as mean +/− s.e.m. using Student’s t-test.

See also Figure S5.