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. 2011 Apr 5;30(11):2101–2114. doi: 10.1038/emboj.2011.104

Figure 4.

Figure 4

Role of TORC1 in autophagy regulation under amino-acid starvation. (A) Wild-type, rho0, Δnpr2, and Δnpr2 rho0 cells expressing prATG8-GFP-ATG8 (upper panel) or prATG8-GFP (lower panel) were exposed to amino-acid starvation medium supplemented with galactose in the absence or presence of rapamycin. Samples were analysed as described in Figure 1A. The means and s.d. of four (n=4) independent experiments are indicated. (B) Wild-type, rho0, Δnpr2, and Δatg7 cells expressing prNPR1-NPR1-HA were exposed to amino-acid starvation medium supplemented with galactose in the absence (upper panels) or presence (lower panels) of rapamycin. The hyperphosphorylated (Npr1-P) and dephosphorylated (Npr1) forms of Npr1 are indicated. Cells were analysed at indicated time points by whole cell extraction and western blot analysis using α-HA and α-Cdc11 antibodies. (C) Wild-type, rho0, Δnpr2, and Δatg7 cells were exposed to amino-acid starvation medium supplemented with galactose. Additionally, wild-type cells were treated with rapamycin during starvation (left panel). Phosphorylated (Atg13-P) and dephosphorylated (Atg13) Atg13 was monitored at indicated time points by whole cell extraction and western blot analysis using α-Atg13 and α-Cdc11 antibodies.