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. 2011 May 3;30(11):2190–2204. doi: 10.1038/emboj.2011.126

Figure 7.

Figure 7

H3K27me and histone acetylation cooperatively regulate stemness in ES cells. (A) Knockdown of Elp3 or Tip60 reverses the effect of Sox2 on Eed-deficient ES cells. Sox2-expressing Eed4 cKO ES cells were transfected with EGFP siRNA (siEGFP), Elp3 siRNA (siElp3) or Tip60 siRNA (siTip60) and cultured with Tet for 2 days. Expression of the indicated genes was examined by quantitative RT–PCR (qRT–PCR). Asterisk, significant difference from EGFP siRNA-transfected cells (P<0.05). (B) Synergistic effect of Eed depletion and HAT knockdown on ES cell differentiation. Wild-type ES cells and Eed4 cKO ES cells were transfected with EGFP siRNA, Elp3 siRNA or Tip60 siRNA, and cultured with or without Tet for 2 days. The expression of the indicated genes was examined by qRT–PCR. Asterisk, significant difference from EGFP siRNA-transfected Eed4 cKO cells cultured with Tet (P<0.05). Hash, significant difference from Elp3 or Tip60 siRNA-transfected Eed4 cKO cells cultured without Tet (P<0.05). In all experiments, error bars represent s.d. (n=3). (C) The proposed role of the Eed/Sox2 regulatory loop in ES cell self-renewal.