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Ribonuclease protection assay of fat-1 transcript levels in cells infected with Ad.GFP (control) and myocytes infected with Ad.GFP.fat-1. Total RNA (10 μg) isolated from the cells was hybridized with antisense RNA probes, digested with ribonuclease, and resolved in denaturing PAGE gel. The fat-1 mRNA was visualized by autoradiography. A probe targeting the β-actin gene was used as a control.
