Figure 1.

Effect of 3ABA on in vitro chemoinvasion. Starved HUVEC were plated in the upper Boyden chamber and treated without and with 3ABA (50 μM) for 6 h. FGF2 was used in the lower compartment as chemoattractant. FGF2 induced a significative increase (+63%) of cells ability to invade Matrigel, compared to unstimulated cells. Treatment with 3ABA significantly inhibited both FGF2-stimulated and spontaneous cell invasiveness of about 55%. Values are expressed as the number of cells migrated through pores, and are mean ± SEM of three experiments. Multiple comparisons were performed by the Student-Newman-Keuls test, after Kruskal-Wallis analysis. (* p < 0.05 compared to control; # p < 0.05 compared to FGF2 stimulated cells).