Table 2.
Expression of PPARγ, COX-2 and iNOS in human HCC cell lines
| PPARγ expression | COX-2 expression | iNOS expression | |||||||
|---|---|---|---|---|---|---|---|---|---|
| IGF-1 | L.obtusiloba extract | IGF-1 and L.obtusiloba extract | IGF-1 | L.obtusiloba extract | IGF-1 and L.obtusiloba extract | IGF-1 | L.obtusiloba extract | IGF-1 and L.obtusiloba extract | |
| HepG2 | 4.31 ± 0.51* | 0.76 ± 0.14 | 1.15 ± 0.09# | n.d. | n.d. | n.d. | 1.17 ± 0.07 | 0.21 ± 0.14* | 0.21 ± 0.09*;# |
| Hep3B | 1.33 ± 0.12* | 0.84 ± 0.09 | 0.81 ± 0.05# | 2.28 ± 0.19* | 0.77 ± 0.08* | 1.09 ± 0.04*;# | n.d. | n.d. | n.d. |
| Huh-7 | 1.17 ± 0.05 | 0.21 ± 0.10* | 0.31 ± 0.12*;# | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| SK-Hep1 | 1.43 ± 0.11* | 0.75 ± 0.09* | 0.89 ± 0.07# | 3.21 ± 0.34* | 0.80 ± 0.07* | 0.82 ± 0.09# | 1.87 ± 0.12* | 0.27 ± 0.12* | 0.35 ± 0.06*;# |
Cell lysates of HCC cell lines as described for Table 1, were subjected to specific western-blots for PPARγ, COX 2 and iNOS and for β actin as equal loading control. Densitometry of specific band intensity was normalized to β-actin expression. Mean values ± SD from three independent experiments. PPARγ, COX-2 and iNOS protein expression levels were calculated in relation to the respective untreated cells. Mean values ± SD from three independent experiments. *P < 0.05 compared to the untreated control, # P < 0.05 compared to IGF-1-treated cells, n.d. - not detected