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. 2011 May 16;9:65. doi: 10.1186/1477-7827-9-65

Figure 2.

Figure 2

UV/Vis spectroscopic cell optimization for MTT and fluorescence technique. Absorbance was measured at 527 nm for MTT and 485 nm for fluorescence. Optimum cell culture conditions: 5-10% CO2, 90% humidity and a constant temperature of 37°C.