HBsAg-specific IgG response to no treatment (NT), vehicle, JVRS-100 (IV, 10 μg), HBsAg (i.m., 5 μg), or HBsAg and JVRS-100 (i.m., 5 μg, 10 were treated A) once every 3 weeks on days 1, 22, and 43 and necropsied on day 57, and B) once every 2 weeks on days 1, 14, 28, 42, 56 and necropsied on day 70. Plasma was assayed for HBsAg-specific IgG. Ten animals were included in each group. (***P ≤ 0.001 using one-way analysis of variance.)