Liver HBV DNA in animals with vehicle, Energix-B (μg), or HBsAg and JVRS-100 (i.m., 5 μg, 10 μg, respectively) in female and male HBV transgenic mice (>6 weeks, 20.2 ± 2.7 g). Animals were treated once every 2 weeks on days 1, 14, 28, 42 and necropsied on day 57. Southern blot analysis and real-time PCR to detect liver HBV DNA has been described (Morrey et al., 2008). The transgene was used as an internal indicator to calculate the pg of HBV DNA per μg of homozygous cellular host DNA. (*P ≤ 0.05, **P ≤ 0.01 compared to HBsAg/JVRS100 group using one-way analysis of variance.)