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. 2011 Jun 17;6(6):e20665. doi: 10.1371/journal.pone.0020665

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Lavra et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Histological pictures of PTC and its matching extra-tumour thyroid tissue immunostained for Gal-3. The upper right panel shows the histological area subjected to laser-based microdissection. (B) Schematic representation of chromosome 7, HIPK2 locus, and regions including the two microsatellite markers and one HIPK2 exon amplified by PCR in cells dissected as in (A). The microsatellite amplification products (left and right panels) were resolved onto 12% PAGE and visualized by silver staining. The arrows indicate the missing bands diagnostic of LOH. Quantitative PCR products amplified with the indicated exon 2 primers were resolved and stained as above (central panel).