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PCR analysis of nymph gut contents for MKα region of pRrMDWK6: positive control (lane 1), gut contents of R. prolixus nymphs, (lanes 2 and 3), and DNA ladder, (lane 4). The MKα region was not present in gut contents of R. prolixus nymphs, indicating failure of gene transfer to wild-type R. rhodnii.
