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Identification of the renatured pET32a(+)/UL55 protein. The renaturation of pUL55 was detected by SDS-PAGE and Western blotting assay using anti-DEV serum for recognization. A. M: Molecular mass marker; Lane 1, the renatured pUL55; Lane 2, the recombinant pUL55 without purification and renaturation. B. M: Prestained Protein Molecular Weight Marker; Lane 1, identified the renatured pUL55 with pre-serum; Lane 2, identified the the renatured pUL55 with the rabbit anti-DEV IgG.
