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Agar diffusion reaction. Agar diffusion reaction was designed for dectecting specificity and sensitivity of anti-pUL55 serum. The result of agar diffusion reaction of the anti-pUL55 serum with purified pUL55 suggested the largest dilution multiple of it was 1:16. 1-5, represented 1:2, 1:4, 1:8, 1:16 and 1:32 diluted anti-seraum, respectively; 6, rabbit pre-immune serum; 7, Purified pUL55.
