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. 2011 May 31;12:276. doi: 10.1186/1471-2164-12-276

Figure 1.

Figure 1

'Stemness' and pluripotency of rESC lines. (A): (a-g): IVF1.2; (h-n): IVF3.2; (o-u): IVF3.3; (a, h, o): Phase-contrast micrograph of an ESC colony on mEFs; (b, i, p): Alkaline phosphatase staining; (c, j, q): Oct-4; (d, k, r): Nanog; (e, l, s): SSEA-4; (f, m, t): TRA-1-60; (g, n, u): TRA-1-81; (B): (a-f): IVF1.2; (g-l): IVF3.2; (m-r): IVF3.3; (a, g, m): Neuronal marker Tuj III expression (green); (b, h) Cardiomyocyte marker α-cardiac actinin (green); (n): Cardiomyocyte marker Troponin C (green); (c, i, o) Endoderm marker α-fetoprotein (AFP, green); (d, j): Neural tube; (p): Squamous epithelium; (e): Cartilage; (k): Muscles; (q): Adipose; (f, l): Intestinal epithelia; (r): Gland. Scale bars: (A: a-u, 100 μm; B: a, g, m, c, i, o, 100 μm; b, h, n, 20 μm; d-f, j-l, p-r, 50 μm).