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. 2011 Jun 17;6(6):e21129. doi: 10.1371/journal.pone.0021129

Figure 1. Assessment of circulating ESO-specific Ab in patients with primary BC.

Figure 1

A. Sera from BC patients and control healthy donors (HD) were screened by ELISA at a serum dilution of 1∶100 on rESO-coated plates and on control plates with no antigen. Samples were considered positive when the optical density (OD) value obtained on rESO-coated plates was higher than the mean+6xSD of OD values obtained with sera from HD on rESO-coated plates. B. Samples that scored positive in A were assessed at a dilution of 1∶400 on plates coated with rESO or rMelan-A. Samples were confirmed as ESO Ab+ if the OD value obtained on rESO-coated plates was both higher than the mean+6xSD of OD values obtained on rMelan-A-coated plates for all patients and 3 folds higher than the OD value obtained for the same serum on rMelan-A-coated plates. C. Serial dilutions of ESO Ab+ sera were assessed on rESO (solid lines) and rMelan-A (dotted lines) coated plates and serum titer was calculated as the serum dilution yielding 50% of maximal OD on rESO-coated plates.