Figure 4. Disrupted membrane permeability and polarity by AgNP/NSP.
Approximately 1×105 E coli DH5α and ML35p strains were spread on NSP (A), AgNP/NSP (B) or silver nitrate (C) containing LB agars, where nanohybrid materials were mixed with at the indicated concentrations. These colonies were counted after overnight incubation at 37°C. After treating E. coli ML35p with the nanomaterials (D, E) or silver nitrate (F) in LB broth, the activity of the released intracellular β-galactosidase in the supernatant was kinetically determined by reacting with ONPG and quantitatively measured with spectrophotometry at 420 nm. The number of fluorescent DiBAC4(3)+ cells in NSP- (G), AgNP/NSP- (H) or silver nitrate- (I) treated E. coli DH5α were determined by FACS analysis.