Figure 8. Hypoxia increases vascular permeability.

Temporal changes in transendothelial resistance across a microvascular endothelial monolayer grown on a Transwell insert treated with vehicle (unstimulated), VEGFA alone, PEDF alone, simultaneous VEGFA+PEDF, PEDF 6 hours post VEGF and PEDF 6 hours post VEGF+γ-secretase inhibitor (γ-SI) under either (a) hypoxia or (b) retinal normoxia. (n = 4 independent experiments). VEGFA and PEDF were used at 100 ng/ml and γ-secretase inhibitor at 1 nM. In the case of PEDF 6 hours post VEGF, Transwell inserts were transferred to new wells containing basal medium without fluorescent dextran. Data are respresented as means± SEM.